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Kalpana, N.
- Transient Neonatal-Onset Hypothyroidism Boosts Estradiol Synthesis in the Testis of Adult Wistar Rat
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Authors
N. S. Venkatesh
1,
M. Kathiresh Kumar
1,
S. Sambavi
1,
N. Kalpana
1,
A. M. Sashi John
1,
J. Anbalagan
1,
R. Ilangovan
1,
M. M. Aruldhas
1
Affiliations
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600 113, IN
1 Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600 113, IN
Source
Journal of Endocrinology and Reproduction, Vol 18, No 2 (2014), Pagination: 95-110Abstract
Transient neonatal hypothyroidism is known to boost Sertoli cell (SC), Leydig cell (LC) and germ cell (GC) number at adult age. Existing reports attribute decreased steroidogenic potential of LCs to unaltered/decreased serum testosterone in such rats, despite a boost in the cell number. However, these studies have ignored the status of estradiol in such conditions. In this present study, we tested the hypothesis "transient neonatal-onset hypothyroidism may lead to a temporal shift in adult rat testicular steroidogenesis towards estradiol production". Hypothyroidism was induced in neonates by providing methimazole (MMI) in drinking water (0.05%) to lactating mothers and pups for a transient period from postnatal day 1 (PND 1) to PND 14 or from PND 1 to PND 29. After the experimental period, the pups were provided drinking water free of MMI and sacrificed on PND 91. Coeval rats without MMI exposure served as control. Radioimmunoassay revealed decreased serum titres of luteinizing hormone (LH), follicle stimulating hormone (FSH), growth hormone (GH) and prolactin (PRL); while serum testosterone remained unaltered, its level in testicular interstitial fluid (TIF) decreased. Between the two major metabolites of testosterone, 5α-dihydrotestosterone (DHT) concentration decreased in serum and TIF, whereas estradiol recorded a significant increase in both. Transient neonatal-onset hypothyroidism decreased 3β-hydroxy steroid dehydrogenase (3β-HSD) in LCs but augmented 17β-HSD activity. Radio-receptor assay revealed decreased concentration of LHR and ER in LCs of hypothyroid groups, whereas AR and PRLR increased. While aromatase activity decreased in LCs, it increased along with FSHR in SCs, when compared to control rats. The changes in LHR and FSHR levels and aromatase were consistent with the expression level of the respective genes. The present study supports the proposed hypothesis and suggests that transient neonatal-onset hypothyroidism-induced boost in estradiol in adult rat testis may be due to augmented expression and activity of aromatase, and FSHR content in SCs of these rats.Keywords
Leydig Cells, Sertoli Cells, LHR, FSHR, Hypothyroidism.
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- Genetics of Medullary Thyroid Carcinoma
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Authors
Affiliations
1 Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
2 Ontogenie et Reproduction, CRBR, CHUL, 2705 Boul W Lauira, Laval University Stc Roy, que, GIV 4G2, CA
1 Department of Endocrinology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai-600113, IN
2 Ontogenie et Reproduction, CRBR, CHUL, 2705 Boul W Lauira, Laval University Stc Roy, que, GIV 4G2, CA
Source
Journal of Endocrinology and Reproduction, Vol 5, No 1&2 (2001), Pagination: 59-76Abstract
Existing modes of diagnosis and therapy for medullary thyroid carcinoma (MTC) have limitations, informations on the genetics of familial medullary thyroid carcinoma (FMTC), a constituent of multiple endocrine neoplasia II (MEN II) syndrome may help to understand the etiology of the disease and to design gene therapy. Association between specific mutations in ret protooncogene to specific phenotypes of MEN II subjects provide vital line of due for the diagnosis of the disease. Screening of suspected/potent carriers of ret (rearranged during transformation) mutations will help to decide about the time and course of treatment. Gene therapy for MTC appears to be imminent and this review attempts to provide a comprehensive account of informations from more than hundred research articles published in this area.Keywords
Calcitonin, Familial Medullary Thyrold Carcinoma, Gene Therapy, Immuno Therapy, Medullary Thyroid Carcinoma, Multiple Endocrine Neoplasia, Ret Protooncogene.- Gestational Exposure to Chromium Alters the Histoarchitecture of Adult Rat Testis: a Light Microscopic Study
Abstract Views :182 |
PDF Views:0
Authors
P. Sekhar
1,
S. Subramanian
2,
N. S. Venkatesh
2,
A. M. J. Sashi
2,
N. Kalpana
2,
J. Anbalagan
2,
G. Vengatesh
2,
P. Govindarajulu
2,
M. A. Akbarsha
3,
M. M. Aruldhas
2
Affiliations
1 Post Graduate Department of Zoology, Voorhees College, Vellore-632001, IN
2 Department of Endocrinology, University of Madras, Dr. ALM Post Graduate Institute of Basic Medical Sciences, Taramani Campus, Chennai-600113, IN
3 Department of Animal Science, Bharathidasan University, Tiruchirappalli-620024, IN
1 Post Graduate Department of Zoology, Voorhees College, Vellore-632001, IN
2 Department of Endocrinology, University of Madras, Dr. ALM Post Graduate Institute of Basic Medical Sciences, Taramani Campus, Chennai-600113, IN
3 Department of Animal Science, Bharathidasan University, Tiruchirappalli-620024, IN